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Gel electrophoresis tank is a versatile laboratory device used in molecular biology and biochemistry for separating, analyzing, and purifying nucleic acids and proteins based on their size and charge.

gel electrophoresis tank

gel electrophoresis tank The gel electrophoresis tank consists of a chamber or tray that holds an agarose gel or polyacrylamide gel, which serves as the separation matrix, and an electrode system that generates an electric field across the gel.

The gel acts as a molecular sieve, allowing the molecules to migrate through it at different speeds depending on their size and charge.

The tank is typically made of a non-conductive material such as acrylic or plastic to prevent electrical shocks and contains a buffer solution that maintains a constant pH and ionic strength during electrophoresis.

The buffer also helps conduct electricity and provides a medium for ion movement.

The gel electrophoresis tank is equipped with two electrodes: a positive electrode (anode) and a negative electrode (cathode).

When an electric current is applied, molecules in the sample are attracted to the opposite charged electrode.

DNA and RNA, which are negatively charged due to their phosphate backbone, migrate towards the positive electrode, while proteins, which can have varying charges, migrate towards the electrode with the opposite charge.

Specifications of gel electrophoresis tank

 Specifications of gel electrophoresis tank The gel inside the tank is cast in a gel tray, which is a rectangular or square-shaped container with wells or slots where the sample is loaded.

The gel is prepared by mixing agarose or polyacrylamide powder with a buffer solution and heating it until it melts.

Once the gel is in a liquid state, it is poured into the gel tray and allowed to solidify, forming a gel matrix with a uniform concentration.

The gel concentration determines the separation range and resolution of the gel electrophoresis. Higher percentage gels (e.g., 2-3%) are used for separating large molecules, such as genomic DNA, while lower percentage gels (e.g., 8-12%) are used for separating smaller molecules, such as PCR products or proteins.

After the gel has solidified, the sample is loaded into the wells using micropipettes or loading tips.

A loading buffer is added to the sample to increase its density and provide a reference point during electrophoresis.

The gel tray is then placed in the gel electrophoresis tank, and the electrodes are connected to a power supply.

buy gel electrophoresis tank

buy gel electrophoresis tank During electrophoresis, the electrical current causes the molecules in the sample to move through the gel.

The rate of migration is determined by the size and charge of the molecules.

Smaller molecules move faster through the gel and travel a greater distance, while larger molecules move slower and have a shorter migration distance.

To visualize the separated molecules, a dye is often added to the gel or the sample.

DNA can be stained with ethidium bromide or other fluorescent dyes that bind to the DNA molecule and fluoresce under ultraviolet light.

Proteins can be stained with Coomassie Brilliant Blue or other protein-specific dyes that produce a color change when bound to proteins.

Once the electrophoresis is complete, the gel is removed from the tank and placed under a UV or visible light source to visualize the separated bands.

The bands can be analyzed and quantified using imaging systems or densitometry software.

gel electrophoresis tank + buy and sell

gel electrophoresis tank + buy and sell Gel electrophoresis tanks come in various sizes and configurations to accommodate different gel sizes and sample volumes.

Mini-gel tanks are used for small-scale separations and are ideal for routine DNA or protein analysis.

Large gel tanks, on the other hand, are used for separating high volumes of samples or for preparative purposes, such as purifying DNA or recovering specific bands of interest.

In addition to standard gel electrophoresis tanks, there are specialized variants available for specific applications.

For example, pulse field gel electrophoresis (PFGE) tanks are designed for separating large DNA fragments using alternating electric fields, while agarose gel electrophoresis tanks with a built-in cooling system are used for temperature-sensitive samples.

In conclusion, the gel electrophoresis tank is an indispensable tool in molecular biology and biochemistry research.

It allows scientists to separate, analyze, and purify nucleic acids and proteins based on their size and charge.

The price of this product is between 20-25 dollars.

With its versatility and ability to handle various sample volumes, the gel electrophoresis tank plays a crucial role in numerous scientific and diagnostic applications.

1. Introduction to Gel Electrophoresis Tanks in Research and Diagnostics